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High-Throughput Continuous Multiplex PCR Device

 

Description

This invention provides high-throughput continuous-flow PCR device, consisting of a flow-through capillary and a cylindrical heating-block assembly. DNA amplification can be achieved by letting a PCR mixture continuously flow through a single channel passing repetitively through three constant temperature zones for melting, annealing, and extension. Also real time monitoring allows to overcome the limitation of conventional PCR device whose limitation is qualitative rather than quantitative. This invention also provides multiple PCR reactions for high-throughput, allowing us to develop a simple but powerful tool for genomics.

Benefits and Applications

Functionality of real time high throughput PCR is demonstrated

Lead small molecules is under development by peptidomimmetic approach

Capillary allows for small amount of sample loading and time-saving process

Easily to interface with DNA microarray for genomics

Intellectual Property Status

Patent pending (PCT)

Related Publication

Analytical Chemistry, 2003, 75, 6029-6033


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